ALOX12 Kit ELISA

N° du produit ABIN2042935

Kit ELISA Humain ALOX12, test Colorimetric pour la quantification de Humain ALOX12.

Aperçu rapide pour ALOX12 Kit ELISA (ABIN2042935)

Antigène: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit ALOX12 Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Marque: BioAssay™

Sensibilité: < 0.116 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Arachidonate-12-Lipoxygenase (ALOX12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Autre désignation: Arachidonate-12-Lipoxygenase (ALOX12)

Pathways: Positive Regulation of Endopeptidase Activity